A Beginner’s Guide to Peptide Reconstitution

Proper reconstitution is essential for maintaining the integrity of lyophilised (freeze-dried) peptides. This guide covers the basics of safely preparing your research compounds for use.

What You’ll Need

• Bacteriostatic Water (BAC Water)
• Sterile syringes (1ml insulin syringes recommended)
• Alcohol swabs
• Your lyophilised peptide vial

Step-by-Step Process

1. Clean the vial tops — Swab both the BAC water vial and peptide vial with alcohol swabs
2. Draw BAC water — Using a sterile syringe, draw your desired amount of bacteriostatic water
3. Add water to peptide vial — Inject slowly down the side of the vial, allowing it to drip gently onto the powder
4. Allow to dissolve — Let the vial sit for 5-10 minutes. Do NOT shake — gently swirl if needed
5. Store correctly — Reconstituted peptides should be refrigerated at 2-8°C

Use Our Mixing Calculator

Not sure how much BAC water to add? Use our Mixing Calculator to determine the exact volume needed for your desired concentration.